kit for the easy and rapid and detection of Brucella spp. This method is highly sensitive and specific and is based on the detection of a DNA-specific fragment through RT-PCR. The test of detection may be carried out in only 45min.

Brucellosis is the most spread zoonosis in the world. It is a high morbidity disease and constitutes a serious social and sanitary problem. It has a systemic activity and may affect nearly every organ in the body, causing a complex clinical syndrome which makes it often difficult to diagnose, especially in non-acute cases. The conventional methods of detection of Brucella spp. involve isolation and eventually culture of the isolated sample. This makes the available methods slow (days or even weeks) and burdensome, with the additional disadvantage of having a relatively low sensitivity and representing a considerable risk of infection for the laboratory staff. It is therefore a need to find new methods of detection of Brucella spp. which overcome the mentioned drawbacks.

The method here offered is a response to the instant needs. It consists of a method of detection of Brucella spp. based on the recognition of a particular DNA-fragment of the pathogen using a RT-PCR (Real Time Polymerase Chain Reaction).

A kit for the easy and rapid diagnosis of Tuberculosis and/or Brucellosis may be designed for carrying out the method.

Advantages of the offer

This new technique is DNA-specific and higly sensitive, thus constituting a very reliable method for the detection of Brucella spp. It further reduces operation times to only 45min. (related to days or even weeks in conventional methods) and is highly suitable for automation, thereby increasing reproducibility and reducing risks of infection of laboratory staff. The nature of the method makes it ideal for being carried out by means of a kit.

Domain of application

The method and the kit proposed may find extensive application in the field of diagnosis, allowing a rapid, easy, and reliable diagnosis of  Brucellosis.

Early Stage

The research group is looking for a collaboration agreement to further develop the technology or a licence agreement.

IPR-Data : P200801280