Out-licensing

New method of cell surface display of single chain antibodies for affinity maturation in a mammalian system

This technology describes a new method of cell surface display of single chain antibodies for affinity maturation in a mammalian system. Cells expressing a rare mutant antibody with higher affinity were enriched about 240 fold by a single-pass cell sorting from a large excess of cells expressing wild-type antibodies with slightly lower affinity. Additionally, a highly enriched mutant with increased binding affinity for CD22 after a single selection of a combinatory library randomizing an intrinsic antibody hotspot was successfully obtained. The system is compatible with other mammalian expression systems and it is a rapid, simple and robust procedure. The method can be useful in isolating high affinity antibodies for cancer, AIDS and other diseases. Applications: A new method of displaying Fvs on human cells; A new method useful to isolate new high affinity antibodies for cancer, AIDS and other diseases Market: The method has a potential several billion dollar market as it can be potentially used in immunotherapeutic approaches for the treatment of Cancer, AIDS and other diseases. Development Status: The technology is currently in pre-clinical stage of development. Publication: M Ho, S Nagata, I Pastan. Isolation of anti-CD22 Fv with high affinity by Fv display on human cells. Proc Natl Acad Sci USA. Jun 20;103(25):9637-9642. Epub 2006 Jun 8, doi 10.1073/pnas.0603653103.

U.S. Provisional Application No. 60/794,212 filed 21 Apr 2006 (HHS Reference No. E-200-2006/0-US-01) Inventors: Ira Pastan and Mitchell Ho (NCI)

Licensees sought: Available for non-exclusive or exclusive licensing. Collaborative Research Opportunity: The National Cancer Institute Laboratory of Molecular Biology is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize Mammalian Cell Surface Display of Fvs for Rapid Antibody Maturation. Please contact Betty Tong, Ph.D. at 301-496-0477 or tongb@mail.nih.gov for more information.